Tissuelyser ii user manual

Sample processing usc school of pharmacy core facilities. Exactive operating manual thermo fisher scientific. Users are strongly advised to not set up experiments and reserve time to use the instrument prior to training or getting access to the lab. Triad qiagen tissuelyser tissue lyser qiagen retsch. Tissuelyser ii qiacube ht qiagility rotorgene q qiaxcel advanced. Dneasy plant handbook the steve clough lab mafiadoc.

Qiagen tissuelyser lt sample disruptor the lab world group. Automated dna extraction from oil palm leaves using. We stiil use pestle and mortar which is very timeconsuming and we would like to speed. Laboratory equipment qiagen tissuelyser ii user manual for disruption of up to 48 or 192 biological samples 48 pages laboratory equipment qiagen ez1 advanced xl user manual. User should provide service requisition form, primary antibody and immuneblanks. Fresh, frozen, or rnalaterallprotect stabilized tissues can be used. User intervention sample lysis samples microtubes are removed from epmotion and sealed with the caps provided. Qiagen kit handbooks and user manuals are available at. Various rat tissues 25 mg each stabilized in allprotect tissue reagent were disrupted using the tissuelyser lt or tissuelyser ii. Do not allow nonstabilized tissues to thaw during weighing or handling prior to disruption in qiazol lysis reagent. Apr 28, 2017 tissuelyser lt tissuelyser lt is a small bead mill that enables low to mediumthroughput sample disruption, of up to 12 samples at the same time. It has been stored under unfavorable conditions for a prolonged period. The ct analyzer user manual or the e book version of the manual has always to be available on the site where the ct analyzer is being used. The qiagen tissuelyser lt sample disruptor provides quick and efficient disruption in up to 12 samples.

For disruption of up to 48 or 192 biological samples 48 pages laboratory equipment qiagen ez1 advanced xl user manual 100 pages laboratory equipment qiagen genereader user manual. The instructions and safety information in the user manual must be followed to ensure safe operation of the tissuelyser ii and to maintain the tissuelyser ii in a. For disruption of up to 48 or 192 biological samples. Tissuelyser lt tissuelyser lt is a small bead mill that enables low to mediumthroughput sample disruption, of up to 12 samples at the same time. Tissuelyser ii medium to highthroughput disruption. The work surfaces of both modules are at the same height and thus the specimens can be transferred easily from one side to the other. Alternatively, a grinding jar set can be used to process large samples. The tissuelyser lt must be used in combination with the tissuelyser lt adapter, 12tube cat. Scope all procedures are applicable to the bcgsc library core and techd groups.

Either fresh, frozen, or lyophilized plant tissue samples can be processed using the tissuelyser. In each of the elements of the a15 analyser, biosystems has used leading edge technology to obtain optimum ana. Friederike kramer, abhishek sharma qiagen gmbh, qiagen strasse 1, 40724 hilden, germany efficient disruption and homogenization. Tissuelyser ii tissuelyser ii delivers medium to highthroughput disruption of up to 2 x 96 samples of tissue, plant material, yeast or bacteria in less than 5 minutes. If the tissuelyser ii is not in use, switch it off at the power switch. Tissuelyser ii the tissuelyser ii system by qiagen disrupts biological samples through highspeed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads. Who uses this guide this exactive operating manual is intended for all personnel that need a thorough understanding of the instrument to perform maintenance or troubleshooting, for example. Applications include dna, rna, and protein release from diverse sample types including plant and animal solid tissue, bacteria, fungi, insects, soil and more. Get the superb tissue quality and proven reliability you expect, plus a safer, more efficient working environment, with the thermo scientific excelsior as tissue processor which provides exceptional and enhanced tissue quality with minimal user interaction. The instructions and safety information in the user manual must be followed to ensure safe operation of the tissuelyser lt and to maintain the tissuelyser lt in a.

The tissuelyser ii simultaneously disrupts multiple biological samples through highspeed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads. Does anyone use the tissuelyser ii qiagen equipment to disrupt organs for virus isolation in cell culture. Excelsior as tissue processor thermo fisher scientific. Perform the maintenance as described in maintenance procedures, page 35. Make sure that the adapter is balanced when fewer than 12 samples are to be processed.

Lt is comparable to that achieved with the wellestablished tissuelyser ii figures 6 and 810. Tissuelyser ii user manual 052016 7 it or the line power cord appears to be damaged. All instructions and protocols in this handbook apply to both the tissuelyser ii and the tissuelyser. Do not allow the tissuelyser adapter plates to come into contact with liquid nitrogen during the procedure.

Tissuelyser ii user manual university of texas at arlington. Tissueruptor, or mortar and pestle followed by qiashredder or needle and syringe use 600. Place the lid of the tissuelyser lt adapter over the insert, and screw the knob until the lid is securely fastened. The kit uses qiagens secondgeneration inhibitor removal technology irt, and is intended for use with. Equipment for tissue disruption and homogenization e. Equipnet is constantly receiving used qiagen equipment through. The instrument is controlled online in real time from an external dedicated pc. For efficient disruption of up to 48 or 192 samples in parallel on the tissuelyser ii. Laboratory equipment qiagen tissuelyser ii user manual. Introduction the a15 analyzer is an automatic random access analyser specially designed for performing biochemical and turbi dimetric clinical analyses. Sample processing usc school of pharmacy core facilities usc.

The user can position the individual modules from either left to right or right to left, depending on personal preference. Tissuelyser ii user manual 042010 7 if the tissuelyser ii becomes electrically unsafe, prevent other personnel from operating it, and contact qiagen technical services. The tissuelyser ii is an integral part of qiagen s complete solution for sample management from sample collection to purification and analysis of dna, rna, and protein. Tissuelyser lt, or tissuelyser ii by referring to the respective user manual and handbook. Rnalater rna stabilization reagent, tissuelyser ii. The tissuelyser lt is compact so you can keep it nearby for easy access. May 2016 tissuelyser ii user manual gtac home page. Tissuelyser adapter set 2 x 96 from qiagen selectscience. View and download qiagen tissuelyser ii user manual online. Operating manuals and protocols department of agronomy.

Nov 20, 2018 steel ball grinding media user manual ninans desktop high speed vibrating ball mill with 50ml, combination of grinding jar and milling ball etc it is the users responsibility, milling media for milling and grinding, steel balls in alumina get price chat online. Triad qiagen tissuelyser tissue lyser qiagen retsch mm300. Storage the tissuelyser lt should be stored upright in a dry environment at 540c. Qiagen offers advanced and innovative solutions for all of your assay and sample equipment needs. Mott center 275 east hancock detroit, michigan 48201 fax. For other disruption devices, refer to suppliers guidelines. Tissuelyser lt tissuelyser ii qiacubedneasy plant mini kit. Qiagen tissuelyser tissue lyser qiagen retsch mm300 qiagen tissuelyser used nice provides rapid and efficient disruption of cells and tissues for the biorobot.

Tissuelyser ii laboratory equipment pdf manual download. Qiagen focuses on breaching great success and development in their company to better serve their consumers. Does anyone use the tissuelyser ii qiagen equipment to. Equipnet is constantly receiving used qiagen equipment through our exclusive contracts with our clients. Bead mill for the disruption of up to 48 samples in tubes or 192 samples in 96well plates. All users of the ct analyzer must read and observe the safety rules described in this section and all relevant installation and operation instructions. Using the appropriate adapter set, up to 48 or 192 samples can be processed at the same time. The tissuelyser ii complements qiagen automation for highthroughput sample preparation and analysis see table qiagen highthroughput automation, such as the qiasymphony sp. Ive read many protocols, help guides and specifically the troubleshooting guide in the kit protocol, but the number one piece of advice im seeing is to use the tissuelyser andor tissueruptor. The tissuelyser ii is also compatible with qiagen manual sample preparation kits see table qiagen purification kits compatible with qiagen disruption systems. For rna applications, stabilization of fresh tissues in rnaprotect tissue reagent prevents rna degradation during sample handling. This small bead mill has high throughput with highspeed shaking for the disruption or homogenization of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads. Processing of up to 2 x 96 samples takes as little as 2 4 minutes. It allows the user to homogenize up to 12 samples at one time.

Qiacube ht manual heparin edta citrate heparin edta 200 50 200 50 200 50 200 50 200 50 200 m1 m2 free up your time. Compact homogenizer for medium to larger sample series adapters 2 x 24 2ml or 2 x 96 1. Automated nucleic acid purification with qiacube ht and the dedicated qiacube ht purification kits saves time and leaves you free to focus on other tasks. See disruption and homogenization using the tissuelyser system, page 15, and centrifugation dneasy 96 procedures, page 17. Refer to the tissuelyser handbook and the centrifuge user manual for operating instructions. Does anyone use the tissuelyser ii qiagen equipment to disrupt organs for virus. Remote instruction will continue through summer session. It has been subjected to severe transport stresses. Access to trlab is granted only after the user has demonstrated the ability to properly operate the instrument without assistant. Does anyone use the tissuelyser ii qiagen equipment to disrupt. Standard operating procedures sop atau panduan instruksi kerja untuk.

Qiagen tissuelyser ii mixer mill grinder pulverizer disruption system. Lung 60 50 40 30 20 10 0 tissuelyser lt tissuelyser ii liver heart brain skin 400 300 200 100 0 heart brainlung tissuelyser lt tissuelyser ii figure 6. Various rat tissues were disrupted using the tissuelyser lt or. Tissuelyser ii user manual 052016 8 the responsible body e. Operating manuals and protocols department of agronomy and. Does anyone have experience with tissue lyser from qiagen. Jul 17, 2011 high throughput homogenizer,can handles up to 96 samples in full walkaway mode. The tissuelyser ii is also compatible with qiagen manual sample preparation kits see table qiagen purification kits compatible with qiagen disruption. Tissuelyser lt handbook qiagen gel electrophoresis. Tissuelyser ii genomicstranscriptomics analysis core. Product use limitations the tissuelyser lt is intended for molecular biology applications. Any license associated with this equipment is nontransferable. Tissueruptor, or mortar and pestle followed by qiashredder or needle and syringe.